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msn_GlutathioneSurvival1
September 17th, 2005, 04:04 PM
<TABLE ><TR><TD><FONT size=2><DIV> <TABLE cellSpacing=0 cellPadding=0 width="100%"> <TBODY> <TR> <TD><SPAN>Br J Cancer.</SPAN> 2001 Mar 23;84(6):844-50.</TD> <TD align=right><SPAN></SPAN></TD></TR></TBODY></TABLE> <DD><FONT size=+1><B>EGCG, a major component of green tea, inhibits tumour growth by inhibiting VEGF induction in human colon carcinoma cells.</B></FONT><BR><BR><A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Jung+YD%22 %5BAuthor%5D"><B>Jung YD</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Kim+MS%22% 5BAuthor%5D"><B>Kim MS</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Shin+BA%22 %5BAuthor%5D"><B>Shin BA</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Chay+KO%22 %5BAuthor%5D"><B>Chay KO</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Ahn+BW%22% 5BAuthor%5D"><B>Ahn BW</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Liu+W%22%5 BAuthor%5D"><B>Liu W</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Bucana+CD% 22%5BAuthor%5D"><B>Bucana CD</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Gallick+GE %22%5BAuthor%5D"><B>Gallick GE</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Ellis+LM%2 2%5BAuthor%5D"><B>Ellis LM</B></A>.<BR><BR>Chonnam University Research Institute of Medical Sciences, Chonnam University Medical School, Kwangju, Korea 501-190.<BR><BR>Catechins are key components of teas that have antiproliferative properties. We investigated the effects of green tea catechins on intracellular signalling and VEGF induction in vitro in serum-deprived HT29 human colon cancer cells and in vivo on the growth of HT29 cells in nude mice. In the in vitro studies, (-)-epigallocatechin gallate (EGCG), the most abundant catechin in green tea extract, inhibited Erk-1 and Erk-2 activation in a dose-dependent manner. However, other tea catechins such as (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epicatechin (EC) did not affect Erk-1 or 2 activation at a concentration of 30 microM. EGCG also inhibited the increase of VEGF expression and promoter activity induced by serum starvation. In the in vivo studies, athymic BALB/c nude mice were inoculated subcutaneously with HT29 cells and treated with daily intraperitoneal injections of EC (negative control) or EGCG at 1.5 mg day(-1)mouse(-1)starting 2 days after tumour cell inoculation. <STRONG>Treatment with EGCG inhibited tumour growth (58%), microvessel density (30%), and tumour cell proliferation (27%) and increased tumour cell apoptosis (1.9-fold) and endothelial cell apoptosis (3-fold) relative to the control condition (P&lt; 0.05 for all comparisons). </STRONG>EGCG may exert at least part of its anticancer effect by inhibiting angiogenesis through blocking the induction of VEGF. Copyright 2001 Cancer Research Campaign.<BR><BR>PMID: 11259102 [PubMed - indexed for MEDLINE] </DD></DIV> <DIV>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx</DIV> <DIV> <TABLE cellSpacing=0 cellPadding=0 width="100%"> <TBODY> <TR> <TD><B> </B><SPAN>Clin Cancer Res.</SPAN> 2004 Jul 15;10(14):4865-73.</TD> <TD align=right><SPAN>&nbsp;</SPAN></TD></TR></TBODY></TABLE> <DD><FONT size=+1><B>Mechanisms of inhibition of tumor angiogenesis and vascular tumor growth by epigallocatechin-3-gallate.</B></FONT><BR><BR><A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Fassina+G% 22%5BAuthor%5D"><B>Fassina G</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Vene+R%22% 5BAuthor%5D"><B>Vene R</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Morini+M%2 2%5BAuthor%5D"><B>Morini M</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Minghelli+ S%22%5BAuthor%5D"><B>Minghelli S</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Benelli+R% 22%5BAuthor%5D"><B>Benelli R</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Noonan+DM% 22%5BAuthor%5D"><B>Noonan DM</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Albini+A%2 2%5BAuthor%5D"><B>Albini A</B></A>.<BR><BR>Tumor Progression Unit and Molecular Oncology Lab, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.<BR><BR>PURPOSE: <STRONG>Green tea consumption has been linked to a reduced occurrence of some tumor types</STRONG>. Current data indicate that the principal mediator of this chemopreventive effect is epigallocatechin-3-gallate (EGCG), the most abundant polyphenol found in dried tea leaves. Here, we examined the effects of this compound on the two key cell populations typically involved in tumor growth: tumor cells and endothelial cells. EXPERIMENTAL DESIGN: The effects of green tea and EGCG were tested in a highly vascular Kaposi s sarcoma (KS) tumor model and on endothelial cells in a panel of in vivo and in vitro assays. RESULTS: <STRONG>EGCG inhibited KS-IMM cell growth and endothelial cell growth, chemotaxis, and invasion over a range of doses; high concentrations also induced tumor cell apoptosis.</STRONG> EGCG inhibited the metalloprotease-mediated gelatinolytic activity produced by endothelial cell supernatants and the formation of new capillary-like structures in vitro. <STRONG>Green tea or purified EGCG when administered to mice in the drinking water inhibited angiogenesis in vivo in the Matrigel sponge model and restrained KS tumor growth. Histological analysis of the tumors were consistent with an anti-angiogenic activity of EGCG and green tea</STRONG>. CONCLUSIONS: <STRONG>These data suggest that the green tea gallate or its derivatives may find use in the prevention and treatment of vascular tumors in a chemoprevention or adjuvant setting</STRONG>.<BR><BR>PMID: 15269163 [PubMed - indexed for MEDLINE] </DD></DIV> <P>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx</P> <P> <TABLE cellSpacing=0 cellPadding=0 width="100%"> <TBODY> <TR> <TD><SPAN>Biol Pharm Bull.</SPAN> 2005 Apr;28(4):574-9.</TD> <TD align=right><SPAN></SPAN></TD></TR></TBODY></TABLE></P> <DD><A target=_top href="http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3580&amp;uid=15802789&amp;db=pubmed&amp;url=h ttp://joi.jlc.jst.go.jp/JST.JSTAGE/bpb/28.574?from=PubMed"></A><FONT size=+1><B>Synergistic effect of green tea catechins on cell growth and apoptosis induction in gastric carcinoma cells.</B></FONT><BR><BR><A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Horie+N%22 %5BAuthor%5D"><B>Horie N</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Hirabayash i+N%22%5BAuthor%5D"><B>Hirabayashi N</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Takahashi+ Y%22%5BAuthor%5D"><B>Takahashi Y</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Miyauchi+Y %22%5BAuthor%5D"><B>Miyauchi Y</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Taguchi+H% 22%5BAuthor%5D"><B>Taguchi H</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Takeishi+K %22%5BAuthor%5D"><B>Takeishi K</B></A>.<BR><BR>Laboratory of Genetic Engineering, Graduate School of Nutritional and Environmental Sciences and School of Food and Nutritional Sciences, University of Shizuoka, Japan.<BR><BR>(-)-Epigallocatechin gallate (EGCG), a major component of green tea catechins, <STRONG>is known to inhibit cell growth and to induce apoptosis in a variety of cultured cells</STRONG>. We examined effects of green tea catechins in cultured cells derived from human gastric carcinoma. The proliferation of four cell lines (MKN-1, MKN-45, MKN-74 and KATO-III) was inhibited with EGCG in a dose-dependent manner. The growth of MKN-45 cells was most efficiently inhibited by the treatment (IC(50): 40 muM EGCG) among the four cell lines, while KATO-III cells were most insensitive (IC(50): 80-150 muM) to the EGCG treatment. In addition, (-)-epicatechin (EC) had a major synergistic effect on the induction of apoptosis in MKN-45 cells treated with EGCG; however it had little effect on the inhibition of cell growth induced by EGCG. To study the molecular mechanisms behind the induction of apoptosis by EGCG, the activity of caspases in MKN-45 cells treated with EGCG was examined. Activity levels of caspases-3, -8 and -9 were elevated in EGCG-treated cells, suggesting that these caspases are involved in the apoptosis induced by EGCG. Furthermore, the synergistic effect of EC with EGCG on the induction of apoptosis was specifically canceled by catalase treatment, suggesting that the <STRONG>synergism involves the extracellular production of reactive oxygen species</STRONG>.<BR><BR>PMID: 15802789 [PubMed - indexed for MEDLINE] </DD> <P>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx</P> <P> <TABLE cellSpacing=0 cellPadding=0 width="100%"> <TBODY> <TR> <TD><SPAN>AIDS.</SPAN> 2002 Apr 12;16(6):939-41.</TD> <TD align=right><SPAN></SPAN></TD></TR></TBODY></TABLE></P> <DD><FONT size=+1><B>Polyphenolic antioxidant (-)-epigallocatechin-3-gallate from green tea as a candidate anti-HIV agent.</B></FONT><BR><BR><A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Fassina+G% 22%5BAuthor%5D"><B>Fassina G</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Buffa+A%22 %5BAuthor%5D"><B>Buffa A</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Benelli+R% 22%5BAuthor%5D"><B>Benelli R</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Varnier+OE %22%5BAuthor%5D"><B>Varnier OE</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Noonan+DM% 22%5BAuthor%5D"><B>Noonan DM</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Albini+A%2 2%5BAuthor%5D"><B>Albini A</B></A>.<BR><BR>Instituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.<BR><BR>Epigallocatechin-3-gallate (EGCG), one of the components of green tea, has been suggested to have antiviral activity. To determine the effects of EGCG on HIV infection, peripheral blood lymphocytes were incubated with either LAI/IIIB or Bal HIV strains and increasing concentrations of EGCG. EGCG strongly inhibited the replication of both virus strains as determined by reverse transcriptase and p24 assays on the cell supernatants.</DD> <P>xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx</P> <P><BR><BR>PMID: 11919502 [PubMed - indexed for MEDLINE] </P></FONT></TD></TR></TABLE>

msn_GlutathioneSurvival1
September 18th, 2005, 05:46 AM
<TABLE ><TR><TD><FONT size=2><DIV> <TABLE cellSpacing=0 cellPadding=0 width="100%"> <TBODY> <TR> <TD><SPAN>Antiviral Res.</SPAN> 2002 Jan;53(1):19-34.</TD> <TD align=right><SPAN><A target=_top href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Display&amp;dopt=pubmed_pubme d&amp;from_uid=11684313"></A><A target=_top></A>&nbsp;</SPAN></TD></TR></TBODY></TABLE> <DD><A target=_top href="http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3048&amp;uid=11684313&amp;db=pubmed&amp;url=h ttp://linkinghub.elsevier.com/retrieve/pii/S0166354201001899"></A>&nbsp;<BR><FONT size=+1><B>Inhibitory effects of (-)-epigallocatechin gallate on the life cycle of human immunodeficiency virus type 1 (HIV-1).</B></FONT><BR><BR><A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Yamaguchi+ K%22%5BAuthor%5D"><B>Yamaguchi K</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Honda+M%22 %5BAuthor%5D"><B>Honda M</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Ikigai+H%2 2%5BAuthor%5D"><B>Ikigai H</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Hara+Y%22% 5BAuthor%5D"><B>Hara Y</B></A>, <A target=_top title="Click to search for citations by this author." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&amp;cmd=Search&amp;term=%22Shimamura+ T%22%5BAuthor%5D"><B>Shimamura T</B></A>.<BR><BR>Department of Microbiology and Immunology, Showa University School of Medicine, 1-5-8, Hatanodai, Shinagawa-ku, 142-8555, Tokyo, Japan. koushi@med.showa-u.ac.jp<BR><BR>Epigallocatechin gallate (EGCg), the major tea catechin, <STRONG>is known as a potent anti-bacterial agent</STRONG>. In addition, <STRONG>anti-tumor promoting, anti-inflammatory, anti-oxidative and antiviral activities have been reported</STRONG>. In the present study, we investigated possible anti-human immunodeficiency virus type-1 (HIV-1) activity of EGCg and its mechanisms of action in the viral life cycle. EGCg impinges on each step of the HIV life cycle. Thus, destruction of the viral particles, viral attachment to cells, post-adsorption entry into cells, reverse transcription (RT), viral production from chronically-infected cells, and the level of expression of viral mRNA, were analyzed using T-lymphoid (H9) and monocytoid (THP-1) cell systems, and antiviral protease activity was measured using a cell-free assay. Inhibitory effects of EGCg on specific binding of the virions to the cellular surfaces and changes in the steady state viral regulation (mRNA expression) due to EGCg were not observed. However, EGCg had a destructive effect on the viral particles, and post-adsorption entry and RT in acutely infected monocytoid cells were significantly inhibited at concentrations of EGCg greater than 1 microM, and protease kinetics were suppressed at a concentration higher than 10 microM in the cell-free study. Viral production by THP-1 cells chronically-infected with HIV-1 was also inhibited in a dose-dependent manner and the inhibitory effect was enhanced by liposome modification of EGCg. As expected, increased viral mRNA production was observed in lipopolysaccharide (LPS)-activated chronically HIV-1-infected cells. This production was significantly inhibited by EGCg treatment of THP-1 cells. In contrast, production of HIV-1 viral mRNA in unstimulated or LPS-stimulated T-lymphoid cells (H9) was not inhibited by EGCg. Anti-HIV viral activity of EGCg may thus result from an interaction with several steps in the HIV-1 life cycle.<BR><BR>PMID: 11684313 [PubMed - indexed for MEDLINE]</DD></DIV></FONT></TD></TR></TABLE>